海洋微生物抗肿瘤活性菌株的分级组合筛选   总被引：8，自引：2，他引：8  

韩小贤  崔承彬  刘红兵  朱天骄  顾谦群  李冬  温江妮 《中国海洋大学学报(自然科学版)》2005,35(1):38-42

为探索如何快速获取活性菌株的筛选模式 ,组合使用海虾生物致死法和tsFT2 10细胞的流式细胞术筛选模型 ,对从青岛前海和胶州湾潮间带海泥、动植物样品中分离的 162株海洋微生物 ,进行了抗肿瘤活性的分级组合筛选。经海虾生物致死法一级初筛 ,得到活性菌株 5 8株 (LC50 <5 0 0 μg/mL) ,阳性率为 3 5 .8%。对初筛有效的 5 8株菌利用流式细胞术筛选模型进行二级复筛 ,得到对tsFT2 10细胞有各种活性的活性菌株共 2 5株 ,阳性率为复筛菌株数的 43 .1%、初筛菌株总数的 15 .4% ,其中 ,诱发细胞坏死伴有凋亡诱导活性的菌株 7株 (占总菌株的 4.3 % ) ,具有细胞周期抑制活性的菌株 18株 (占总菌株的 11.1% )。该分级组合筛选模式与流式细胞术筛选模型的单独筛选模式相比 ,无漏筛 ,具有成本低、速度快、宜于大规模筛选等特点。  相似文献   

高产壳聚糖酶菌株的筛选和发酵产酶条件研究   总被引：12，自引：1，他引：12  

逄玉娟  韩宝芹  刘万顺  杨菊林 《中国海洋大学学报(自然科学版)》2005,35(2):287-292

为探讨专一性地降解甲壳质或壳聚糖的甲壳质酶或壳聚糖酶 ,从采集的土样中分离到 4株产壳聚糖酶能力较强的菌株 ,经摇瓶复筛 ,菌株YJ0 2产酶能力最强。对其发酵产酶条件的研究结果表明其产酶最适培养基组分为 (% ,w/v) :粉末壳聚糖 2 .0 ,葡萄糖 0 .1,NH4NO3 1.0 ,酵母提取物 0 .5 ,K2 HPO40 .0 7,KH2 PO40 .0 3 ,NaCl0 .5 ,MgSO4·7H2 O 0 .0 5 ,起始 pH 6.0。最适产酶培养条件是 :5 0 0mL三角烧瓶装瓶量为 15 0mL ,2 .0 % (v/v)接种量 ,3 0℃ ,15 0r/min培养 72h。在最适产酶培养条件下 ,72h时菌株YJ0 2发酵液中壳聚糖酶活力可达到 17.0 4U/mL。  相似文献   

Towards the identification of warning criteria: Analysis of a ship accident database   总被引：3，自引：0，他引：3  

A. Toffoli  J.M. Lefvre  E. Bitner-Gregersen  J. Monbaliu 《Applied Ocean Research》2005,27(6):281-291

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The status of geological dredging techniques     

R. B. Kidd  Q. J. Huggett  A. T. S. Ramsay 《Marine Geophysical Researches》1990,12(1-2):131-143

Scientific sea-floor dredging is currently used in marine geology primarily by the hard-rock community interested in the recovery of basement rock samples from the unsedimented deep ocean floor. The technique has generally been eclipsed by ocean drilling for recovery of sedimentary rocks, because of perceived uncertainties in the location of sampling and in the representativeness of recovered material. This contribution reviews dredging equipment currently in use by marine geological institutions and refers to pinger attachments that allow precise information on the behaviour of the dredge to be telemetered back to the ship. We argue that improvements in ship navigation and transponder navigation at the seafloor, when used in conjunction with surface and/or deeply towed sidescan and swathemapping surveys, now allow for considerably less uncertainty on the location of dredge sampling. Refined sorting criteria for dredge hauls are now also available. Recent comparisons of regional sample recovery by ocean drilling and by dredge sampling indicate that the dredge hauls can usefully supplement the drilling data in the construction of sedimentary and tectonic histories of seafloor areas.  相似文献   

ENVISAT-1卫星测高数据编辑标准的研究   总被引：2，自引：0，他引：2  

赵小阳  李建成  金涛勇  褚永海 《海洋测绘》2006,26(6):65-67

介绍了环境卫星(ENVISAT-1)的基本情况及其主要技术参数,在借鉴其他卫星测高数据编辑标准和大量统计基础上,制定了ENVISAT-1卫星的数据编辑标准,包括冰标志和S波段异常的确定,并给出了各改正项合理的限值。  相似文献   

Structural modeling and testing of a concrete canoe     

F. Paradis  G. Gendron 《Ocean Engineering》2007,34(1):206-217

A finite element model is set up and experimental tests are performed to help understand the behavior of a concrete canoe and subsequently optimize its design. First, the performance criteria that must be satisfied to participate at the annual ASCE/Master Builders competition are described. Then, the finite element model and the different loading cases that were studied are presented. Results from these loading cases are discussed and used to optimize the material properties as well as the thickness of the hull and the dimensions of the gunwale and reinforcing ribs. Static and dynamic experimental tests were also conducted to validate the results of the finite element analyses. The results indicate that the main stresses and strains are caused by the static load cases. The additional stresses caused during races are small.  相似文献   

An evaluation of systems for the integrated assessment of capture fisheries     

Duncan Leadbitter  Trevor J. Ward 《Marine Policy》2007

Integrated fishery assessments are intended to take an integrated and wholistic view of fisheries management, to provide comprehensive systems-based evaluations of the sustainability of individual fisheries and their products. Using guidance derived from decision theory and management science, we develop 18 criteria to evaluate the likely effectiveness of nine integrated fishery assessment systems. The assessment systems do not consistently consider four key criteria: the socio-economic impacts of fisheries, the provision of food security, the local relevance of the assessment to the fishery under assessment, and the independent peer review of assessment outcomes. Other important areas of weakness include the lack of appropriate consultation processes relating to both the design of the assessment system and the outcomes of fishery assessments, and limitations in the quality of data and information used in assessments. Improvements will be needed in these areas across all the systems we evaluated if fishers, consumers, managers and other stakeholders are to be provided with consistently reliable assessment outcomes, and an assurance of the sustainability of capture fisheries.  相似文献   

Examination of empirical formulas for wave shoaling and breaking on steep slopes   总被引：1，自引：0，他引：1  

Ching-Piao Tsai  Hong-Bin Chen  Hwung-Hweng Hwung  Ming-Jen Huang 《Ocean Engineering》2005,32(3-4):469-483

Estimation of the wave height transformation of shoaling and breaking is essential for the nearshore hydrodynamics and the design of coastal structures. Many empirical formulas have been well recognized to the wave height transformation, but most of them were only applicable for gentle slopes. This paper reports the experimental results of wave shoaling and breaking over the steep slopes to examine the applicability of the previous empirical formulas. Two steep bottom slopes of 1/3 and 1/5, and one gentle slope of 1/10 were conducted in the present experiments. Experimental results show that the shoaling distance of steep slopes become short and the surface waves may be partially reflected from the steep bottom, thus the estimation of wave shoaling using the well-known previous formula did not conform completely to the experimental results. The previous empirical formulas for the wave breaking criteria were also examined, and the modified equations to the steep beaches were proposed in this work. A numerical model was finally adopted to calculate the wave height transformation in the surf zone by introducing the modified breaking index.  相似文献   

基于OWA的大理市土地生态安全评价研究   总被引：4，自引：1，他引：3  

张洪  王安琦  宋贝扬 《地理科学》2017,37(11):1778-1784

从自然生态环境角度出发,从自然因子、生态环境因子、景观因子3个层面选取16项评价指标建立大理市土地生态安全评价体系,并运用IDRISI软件中的MCE模块进行有序加权平均算子（OWA）多准则决策评价,得到不同决策风险系数下的大理市土地生态安全评价结果。结果显示,由于决策者风险态度不同,得到土地生态安全评价结果差异较大,在实际应用中,必须结合研究区的实际情况以及规划的目的选择不同的风险系数进行评价,才能得到更加真实有效的结果。  相似文献   

Failure Pressure Analysis of Corroded Moderate-to-High Strength Pipelines          下载免费PDF全文

苏晨亮  李昕  周晶 《海洋工程》2016,(1):69-82

Based on the elastic-plastic, large-deformation finite element method, burst capacity of steel pipeline with longitudinal corrosion defect subjected to internal pressure is studied. The appropriate stress-based criterion is used to predict the failure pressure of finite element model of corroded pipeline under internal pressure. By considering the pipe steel grades and geometries of corrosion defects, a series of finite element analyses is conducted. The effects of corrosion depth, length and width on burst capacity are also discussed. A specific failure pressure solution for the assessment of corrosion defects in moderate-to-high strength pipeline is proposed on the base of numerical results. The failure pressures predicted by the proposed method are in better agreement with the experimental results than the results by the other methods.  相似文献